Q5
Single choice
In an assay for the presence of a specific disease gene alelle in several individuals, you isolate genomic DNA from each and perfom polymerase chain reaction (PCR) using gene-specific primers. The PCR product is expected to have a recognition site for the restriction endonuclease BamHI. Following PCR and BamHI digestion, the products are separated by gel electrophoresis and the results are shown in below figure.
Which lane corresponds to the individual demonstrating heterozygosity for the BamHI site?
