ASCP ASCP-MLT Online Practice Questions and Exam Preparation

ASCP ASCP-MLT Online Questions & Answers

• Question 301:

  Gating sets up boundaries around a subset of data points to segregate those events for analysis.

  Gating the proper cell population is crucial for flow cytometric analysis. The CD marker information presented on the histogram data is only representative of the cells inside the gate.

  When analyzing the raw data of a sample on the flow cytometer, CD marker information on the histograms represents data inside the _____ population.

  A. Gated
  B. Non-gated
  A. Gated

• Question 302:

  Antibodies in the Rh system typically exhibit which one of the following characteristics?

  A. Reacts best at 37°C and AHG
  B. Reacts best at room temperature
  C. Shows hemolysis better than agglutination
  A. Reacts best at 37°C and AHG

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  Explanation/Reference:

• Question 303:

  The correct response is option B: The Hepatitis B "e" Antigen (HBeAg). This antigen indicates the virus is actively replicating and therefore the patient is very infectious. The hepatitis B "e" antigen is present when the virus is actively replicating. In cases of unintentional needlesticks, infectivity is of highest concern. The risk for infection is greatest during phases of increased HBeAg serology. The Hepatitis B surface antigen is the first detectable marker, but if the patient is known to have Hepatitis B already, it would be relatively unhelpful to confirm the condition with another HBsAG test. The core antigen is not detectable because it is covered by the nuclear envelope. Antibody response patterns would not be very helpful either as the patient has already been diagnosed with acute Hepatitis B. IgG antibodies would indicate recovery, which is not the case for this patient and IgM antibodies indicating a recent or acute infection would only confirm what is already known. Recall, in cases of unintentional needlesticks, infectivity is of highest concern.

  A phlebotomist at a local hospital recently had an accidental needle stick while drawing blood from a patient being treated for acute hepatitis B. Which serological marker from the patient would be of most value to the physician evaluating the phlebotomist's possible infection status?

  A. Hepatitis B surface Antigen (HBsAg)
  B. Hepatitis B e Antigen (HBeAg)
  C. Hepatitis B core Antigen (HBcAg)
  D. Anti-Hepatitis B e (anti-HBe) IgM
  E. Anti-Hepatitis B core (anti-HBc) IgG
  B. Hepatitis B e Antigen (HBeAg)

• Question 304:

  The federal government has categorized critical biological agents into three groups: A, B, and C. Those agents in category A are highest-priority because they can be easily disseminated or transmitted person-to-person.

  The federal government has categorized critical biological agents into three groups: A, B, and C. Those agents in category A are highest-priority because they:

  A. Cause moderate morbidity and low mortality.
  B. Could be engineered for mass dissemination at some future date.
  C. Can be easily disseminated or transmitted person-to-person.
  C. Can be easily disseminated or transmitted person-to-person.

• Question 305:

  Cerebrospinal fluid has three main functions:

  Protect brain and spinal cord from trauma.

  Supply nutrients to nervous system tissue.

  Remove waste products from cerebral metabolism.

  Which of the following are functions of CSF? Please select all correct answers

  A. Supply nutrients to nervous system tissue.
  B. Regulate body metabolism.
  C. Protect spinal cord from injury.
  D. Remove waste products from cerebral metabolism.
  A. Supply nutrients to nervous system tissue.
  C. Protect spinal cord from injury.
  D. Remove waste products from cerebral metabolism.

• Question 306:

  Alpha-fetoprotein is a substance typically used in the triple test during pregnancy and for screening chronic liver disease patients for hepatocellular carcinoma. Increased concentrations of alpha-fetoprotein (AFP) in adults are MOST characteristically associated with:

  A. hepatocellular carcinoma
  B. alcoholic cirrhosis
  C. chronic active hepatitis
  D. multiple myeloma
  A. hepatocellular carcinoma

• Question 307:

  A combination of (nonselective) 5% sheep blood and (selective) MacConkey agars is sufficient for the recovery of the pathogenic microorganisms that are most commonly encountered in urinary tract infections (UTIs). MacConkey is the

  selective culture medium that is most commonly used to inhibit growth of gram-positive organisms (most UTIs are caused by gram-negative organisms).

  Eosin methylene blue (EMB) is a selective agar that also inhibits the growth of gram- positive organisms. Therefore, using only a combination of MacConkey and EMB would prevent the detection of a gram-positive organism, if this were the

  cause of the infection.

  Chocolate agar or other enriched media may be needed in addition to blood and MacConkey if a more fastidious organism is suspected.

  Thayer-Martin would be used specifically for recovery of Neisseria gonorrhoeae. Thayer- Martin (or Modified Thayer-Martin) inhibits other microorganisms and allows the selective recovery of both N. gonorrhoeae and N. meningitidis.

  Microbiology

  Which culture agar combinations below will usually be sufficient for MOST routine urine culture investigations?

  A. 5% sheep blood and Chocolate
  B. 5% sheep blood and MacConkey
  C. 5% sheep blood and Thayer-Martin
  D. MacConkey and Eosin Methylene Blue
  E. Thayer-Martin and Chocolate
  B. 5% sheep blood and MacConkey

• Question 308:

  Haemophilus influenzae is both X and V factor dependent for growth, and is the most common cause of obstructive laryngitis and epiglottitis. H. parainfluenzae is only V-factor dependent and causes only mild pharyngitis, rarely obstructive in nature. H. aphrophilus can be part of the normal upper respiratory flora in humans, does not cause pharyngitis, and is not X or V-factor dependent for growth. Although H. haemolyticus is both X and V- factor dependent, it is beta hemolytic on blood agar and is generally considered non pathogenic.

  Acute obstructive epiglottitis, both in adults and children, is caused by the bacterial species shown in this split screen photograph. The colonies recovered on chocolate agar (upper frame) required both hematin and NAD, (as shown by colony growth only between the X and V strips in the lower frame). The most likely identification is:

  

  A. Haemophilus parainfluenzae
  B. Haemophilus influenzae
  C. Haemophilus aphrophilus
  D. Haemophilus haemolyticus
  B. Haemophilus influenzae

• Question 309:

  The laboratory employee with a 4-year college degree who performs clinical analysis is the:

  A. clinical laboratory assistant (CLA)
  B. phlebotomist
  C. clinical laboratory scientist (CLS) or medical laboratory scientist (MLS) in newer terminology or Medical Technologist (MT) in older terminology
  D. medical laboratory technician (MLT)
  C. clinical laboratory scientist (CLS) or medical laboratory scientist (MLS) in newer terminology or Medical Technologist (MT) in older terminology

• Question 310:

  HbsAg is positive in acute and chronic Hepatitis B infections, since the antigen is found on the actual surface of the virus. HbeAg is present in the blood when the hepatitis B viruses are replicating, indicating an active infection. Anti-Hbc IgM is present due to the immune response to the presence of the hepatitis core antigen and indicates an acute infection. Anti-HBs is generally interpreted as indicating recovery and immunity from hepatitis B virus infection, according to the CDC. Given the following results, what is the immune status of the patient? HbsAg: positive HbeAg: positive Anti-HBc IgM: positive Anti-HBs: negative

  A. acute infection
  B. chronic infection
  C. immunization
  D. susceptible
  A. acute infection